Posted 24 April 2018 - 07:27 AM
Posted 24 April 2018 - 08:32 AM
Depends on the protocol, but 10 ul ligation in 50 ul of cells is probably too much. Ideally you want 5% or less ligation mix in your cells. Having said that, I would be surprised if you didn't get at least some colonies.
The cells stored at -20 will probably not work once thawed.
If you have some ligation stored, that should work, even if it is less than 1 ul.
Posted 27 April 2018 - 09:55 AM
And for transformation of that stored in-20, if I directly go to heat shock and continue the protocol? Any chance?