Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to estimate MOI for a complex cell type

virus moi infection

  • Please log in to reply
No replies to this topic

#1 MichaelXu

MichaelXu

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 21 April 2018 - 05:48 PM

Hi, all

I am currently confused about the estimation of multiplicity of infection (MOI), here is an example in a scientific article

in figure 3b, they tested the virus, and get estimated MOI based on this:

 

 

To estimate the MOI, we isolated a total of 45 individual neurons from three animals injected with MAPseq virus into the right LC and sequenced the barcodes within each neuron. On average, infected LC neurons contained 1.2 ± 0.1 barcodes each, implying MOI of 0.43 (Figure 3B).

 

Thanks in advance!







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.