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How to estimate MOI for a complex cell type

virus moi infection

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#1 MichaelXu



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Posted 21 April 2018 - 05:48 PM

Hi, all

I am currently confused about the estimation of multiplicity of infection (MOI), here is an example in a scientific article

in figure 3b, they tested the virus, and get estimated MOI based on this:



To estimate the MOI, we isolated a total of 45 individual neurons from three animals injected with MAPseq virus into the right LC and sequenced the barcodes within each neuron. On average, infected LC neurons contained 1.2 ± 0.1 barcodes each, implying MOI of 0.43 (Figure 3B).


Thanks in advance!

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