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# Papain activity calculation

papain activity calculation

5 replies to this topic

Newbie !!

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Posted 20 April 2018 - 12:46 PM

Hi,

I am confused as how to calculate the activity of Papain.

It's in lyophilized form and I want to prepare 200 µl. So, how can I calculate the activity.

I have the following information: Size - 1 u/vial and Act: 26.1 u/mgP; 57% protein

What should I dissolve it in to prepare the solution?

Thanks

Cheers,

### #2 bob1

bob1

Thelymitra pulchella

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Posted 20 April 2018 - 01:14 PM

Do you have an activity that you want? Or do you want activity per ul?

If the latter, then it is simple 1 U/200 ul = 0.005 U/ul

Newbie !!

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Posted 20 April 2018 - 01:15 PM

Do you have an activity that you want? Or do you want activity per ul?

If the latter, then it is simple 1 U/200 ul = 0.005 U/ul

Hi Bob,

I want an activity of 20 units/ml. How do I calculate that?

Cheers,

### #4 bob1

bob1

Thelymitra pulchella

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Posted 20 April 2018 - 07:42 PM

200 ul is 0.2 of a ml.

For this you can use the n=c*v

n= 20 u/ml * 0.2 ml

n=4 U

Newbie !!

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Posted 21 April 2018 - 06:45 AM

200 ul is 0.2 of a ml.

For this you can use the n=c*v

n= 20 u/ml * 0.2 ml

n=4 U

So, how much of the solution (example, PBS) should I add to the lyophilized powder to make it 4U? Also, is PBS is the best solution to dissolve the Papain or do you recommend dissolving it in any other solution?

Cheers,

### #6 bob1

bob1

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Posted 21 April 2018 - 08:48 PM

Your vial contains 1 U total... so you would add 1/20th of a ml or 50 ul. You can not make a 200 ul solution with a concentration of 20 U/ml with this vial alone.