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Papain activity calculation

papain activity calculation

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5 replies to this topic

#1 Mad Researcher

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Posted 20 April 2018 - 12:46 PM

Hi,

I am confused as how to calculate the activity of Papain. 

It's in lyophilized form and I want to prepare 200 µl. So, how can I calculate the activity.

I have the following information: Size - 1 u/vial and Act: 26.1 u/mgP; 57% protein

 

What should I dissolve it in to prepare the solution?

 

Thanks


Cheers,

Mad Researcher

#2 bob1

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Posted 20 April 2018 - 01:14 PM

Do you have an activity that you want? Or do you want activity per ul? 

 

If the latter, then it is simple 1 U/200 ul = 0.005 U/ul



#3 Mad Researcher

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Posted 20 April 2018 - 01:15 PM

Do you have an activity that you want? Or do you want activity per ul? 

 

If the latter, then it is simple 1 U/200 ul = 0.005 U/ul

Hi Bob,

I want an activity of 20 units/ml. How do I calculate that?


Cheers,

Mad Researcher

#4 bob1

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Posted 20 April 2018 - 07:42 PM

200 ul is 0.2 of a ml. 

 

For this you can use the n=c*v

 

n= 20 u/ml * 0.2 ml

n=4 U



#5 Mad Researcher

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Posted 21 April 2018 - 06:45 AM

200 ul is 0.2 of a ml. 

 

For this you can use the n=c*v

 

n= 20 u/ml * 0.2 ml

n=4 U

So, how much of the solution (example, PBS) should I add to the lyophilized powder to make it 4U? Also, is PBS is the best solution to dissolve the Papain or do you recommend dissolving it in any other solution?


Cheers,

Mad Researcher

#6 bob1

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Posted 21 April 2018 - 08:48 PM

Your vial contains 1 U total... so you would add 1/20th of a ml or 50 ul. You can not make a 200 ul solution with a concentration of 20 U/ml with this vial alone.





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