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Should Lineage marker cocktail be used in cell lines to remove lineage specific

Cell Culture FACS CellLines Lineage markers

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#1 rv1234

rv1234

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Posted 18 April 2018 - 10:07 PM

Hi,

 

I am isolating some specific sub-populations from breast cancer cell lines. The biomarkers for which I already have. I would also like to investigate the cells which are not unstained by these biomarkers, I do not want to target differentiated cells as my target are stem cells. My question is, is it advisable to use Lineage specific markers (CD3, CD14, CD31, CD41, CD19, CD56) along with the specific markers or are Lineage specific Markers not needed? Because these Lineage markers are mostly for immune and blood cells and as far as i understand a mammary cell line like MCF-7 wont contain any.

 

Thanks

 







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