Hi all, just learned that PFX from Invitogen has been discontinued. We've been good friends for a long time, and I'm pretty sad about it
I looked into the replacement polymerase, SuperFi, but I'm not at all happy that it is a master mix. How am I supposed to troubleshoot when I have so little control over what goes into my reaction? I'm looking for a new high fidelity, friendly polymerase that works well for routine PCR-based subcloning with separate kit components. What do you all like best? Thanks!