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Favorite polymerase for general cloning?


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3 replies to this topic

#1 DrSnood

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Posted 17 April 2018 - 11:28 AM

Hi all, just learned that PFX from Invitogen has been discontinued. We've been good friends for a long time, and I'm pretty sad about it :(

 

I looked into the replacement polymerase, SuperFi, but I'm not at all happy that it is a master mix. How am I supposed to troubleshoot when I have so little control over what goes into my reaction? I'm looking for a new high fidelity, friendly polymerase that works well for routine PCR-based subcloning with separate kit components. What do you all like best? Thanks!



#2 bob1

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Posted 17 April 2018 - 12:46 PM

I've used KOD in the past. Plenty of people use Pfu



#3 Tristen

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Posted 30 May 2018 - 08:39 PM

Phusion from either NEB or Thermo has worked consistently for me.



#4 OldCloner

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Posted 08 June 2018 - 11:49 AM

iProof from BioRad and G5 DNA polymerase from NEB have worked well for me.






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