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Favorite polymerase for general cloning?

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#1 DrSnood



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Posted 17 April 2018 - 11:28 AM

Hi all, just learned that PFX from Invitogen has been discontinued. We've been good friends for a long time, and I'm pretty sad about it :(


I looked into the replacement polymerase, SuperFi, but I'm not at all happy that it is a master mix. How am I supposed to troubleshoot when I have so little control over what goes into my reaction? I'm looking for a new high fidelity, friendly polymerase that works well for routine PCR-based subcloning with separate kit components. What do you all like best? Thanks!

#2 bob1


    Thelymitra pulchella

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Posted 17 April 2018 - 12:46 PM

I've used KOD in the past. Plenty of people use Pfu

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