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Outer membrane protein extraction


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#1 Siniya

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Posted 17 April 2018 - 07:14 AM

I couldn't get essential bands by SDS PAGE of outer membrane protein. What is the esssential criteria to get all required bands of outer membrane protein ? If i place whole cell protein in 1% of sodium lauryl sarcosine , overnight, the outer membrane protein degrades? Or by this is it possible to extract properly all minute outer membrane proteins

#2 mdfenko

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Posted 23 April 2018 - 02:56 PM

the sarcosine should not degrade the protein, it should disrupt the membrane. there are, however, proteases that will be released and they may degrade the proteins.

 

do you add a protease inhibitor cocktail to your extraction medium?


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genius does what it must
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