
Outer membrane protein extraction
#1
Posted 06 April 2018 - 03:59 AM
#2
Posted 06 April 2018 - 05:30 AM
membrane proteins usually, and mostly, will pellet with the membrane fragments when centrifuged. you would probably be able to extract them from the pellet, rather than the supernate.
talent does what it can
genius does what it must
i used to do what i got paid to do
#3
Posted 06 April 2018 - 05:52 AM
#4
Posted 06 April 2018 - 05:54 AM
#5
Posted 09 April 2018 - 04:22 PM
if you lysed the tissue with detergent then the supernate should contain membrane proteins.
some proteins don't tolerate freezing. you may have to clarify after thawing before further extraction. but, just follow the procedures that you have.
talent does what it can
genius does what it must
i used to do what i got paid to do
#6
Posted 11 April 2018 - 09:03 PM