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Outer membrane protein extraction


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5 replies to this topic

#1 Siniya

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Posted 06 April 2018 - 03:59 AM

Can we extract outer membrane protein from stored whole cell protein supernatant ?

#2 mdfenko

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Posted 06 April 2018 - 05:30 AM

membrane proteins usually, and mostly, will pellet with the membrane fragments when centrifuged. you would probably be able to extract them from the pellet, rather than the supernate.


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#3 Siniya

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Posted 06 April 2018 - 05:52 AM

Actually, while reading the procedure of both whole cell protein(WCP) and outer membrane protein(OMP), OMP follows the WCP extraction procedure initially.Later OMP is extracted after some additional steps. Generally the extracted WCP is stored at -20?C. So i think we can extract OMP from WCP after following the additional steps. Is it right ?

#4 Siniya

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Posted 06 April 2018 - 05:54 AM

Also, WCP is stored AS SUPERNATANT.

#5 mdfenko

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Posted 09 April 2018 - 04:22 PM

if you lysed the tissue with detergent then the supernate should contain membrane proteins.

 

some proteins don't tolerate freezing. you may have to clarify after thawing before further extraction. but, just follow the procedures that you have.


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#6 Siniya

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Posted 11 April 2018 - 09:03 PM

Thankyou sir




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