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Standardization of SDS-PAGE!!!


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#1 Siniya

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Posted 04 April 2018 - 08:32 AM

What is meant by standardization of SDS-PAGE?
How to do that ?

#2 Thomson

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Posted 04 April 2018 - 04:20 PM

Maybe it meant to use consistent host protein like beta actin etc for normalization



#3 mdfenko

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Posted 05 April 2018 - 08:40 AM

you run size standards with each gel (more than one, non-adjacent lane for more accuracy) to which you can compare your sample.

 

it may also refer to standardization of running conditions (percentage and composition of acrylamide, length of running gel, stacking gel, sample volume, volt-hours, etc).


Edited by mdfenko, 05 April 2018 - 08:43 AM.

talent does what it can
genius does what it must
i do what i get paid to do




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