I try to characterize expression of some enzymes in skeletal muscle in control and experimental samples from mice.
I started this research as a small experiment some years ago in another laboratory, I got convincing results, which were checked by more competent colleagues, and now I expand this study in my laboratory.
Most of the chemicals and the primer sequences are the same, mice are ICR like before.
Before going further I decided to reproduce the previous experiment.
I was shocked to see amplification product in my control samples, where there shouldn`t be anything. Absolutely anything.
I was hypershocked to see no or very low amplification in my experimental samples, which is opposite to my previous results.
Could anybody possibly have an idea why is this happening?