Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

ELISA and Sugar/saccharides

ELISA Sugar Saccharide inhibitory

  • Please log in to reply
1 reply to this topic

#1 Rnotk



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 101 posts

Posted 31 March 2018 - 09:31 AM

Hi All


Does anyone know sugar or mono/poly saccharide in sample affect/interfere the ELISA result or prevent antibody/target binding?

This ELISA is not to measure the saccharide but measuring something else in the sample but the sample contains polysaccharides.


Due to the experimental design, this polysaccharide cannot be removed, and the sample cannot be diluted.


Please let me know if there is any publication or data suggesting this issue and possible solution for this assay to work.


Thank you in advance

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,383 posts

Posted 05 April 2018 - 08:49 AM

while they shouldn't effect the elisa, you can determine this empirically by running a range of the polysaccharides to determine their effect.

talent does what it can
genius does what it must
i used to do what i got paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.