I have a protein which binds Src under non-stimulated conditions, upon EGF stimulation there appears to be a dissociation between the protein and Src. Mass Spec phosphorylation data shows an EGF-stimulated phospho site in a proline rich region which may contain a potential Src SH3 binding site. How could I determine if the phosphorylation site is responsible for the dissociation from Src?
Phosphorylation site and Src
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