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Phosphorylation site and Src


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#1 joek1

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Posted 30 August 2004 - 06:25 AM

I have a protein which binds Src under non-stimulated conditions, upon EGF stimulation there appears to be a dissociation between the protein and Src. Mass Spec phosphorylation data shows an EGF-stimulated phospho site in a proline rich region which may contain a potential Src SH3 binding site. How could I determine if the phosphorylation site is responsible for the dissociation from Src?
Thanks.

#2 Simonsays

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Posted 31 August 2004 - 12:00 PM

Hi!
I'd try directed mutagenesis of the residue for a glycine or an alanine... Express your protein and see if Src is still able to bind it under basal conditions

How about it?

Simon




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