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Preparation, Purification and Identification of Lysozyme (Part Three)


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#1 biomart



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Posted 25 March 2018 - 07:00 PM

1.2 Gel separation of lysozyme

Gel filtration, also known as molecular sieve filtration, or exclusion chromatography. Its outstanding advantage is that the gels used in the chromatography are inert carriers, having no charge, weak adsorption force, mild operating conditions, and can be carried out over a wide temperature range. In the meantime, organic solvents are not needed. It has a good separation effect for the polymer material. Gel is a three-dimensional network structure and porous insoluble bead-like particulate material. It is used to separate substances, mainly based on the porous gel with different radius of the protein molecules (nearly spherical) with different exclusion effect achieved. That is, it is based on the molecular size of the physical properties of the separation and purification.

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