This part is to give an explanation about the first step — Preparation of lysozyme. And the next post gonna talk about the gel separation of lysozyme.
1. Experimental methods and steps:
This experiment mainly utilized 724 acid cation exchange resin adsorption, (NH4) 2SO4 salting-out, ph adjustment isoelectric point removal of hybrid protein, gel layer sucked separation and purification, and finally the purified product was identified by SDS-PAGE.