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Preparation, Purification and Identification of Lysozyme (Part Two)

Lysozyme

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#1 biomart

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Posted 25 March 2018 - 06:58 PM

This part is to give an explanation about the first step — Preparation of lysozyme. And the next post gonna talk about the gel separation of lysozyme.

Experimental steps

1. Experimental methods and steps:

This experiment mainly utilized 724 acid cation exchange resin adsorption, (NH4) 2SO4 salting-out, ph adjustment isoelectric point removal of hybrid protein, gel layer sucked separation and purification, and finally the purified product was identified by SDS-PAGE.

More at Preparation, Purification and Identification of Lysozyme (Part Two)


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