I have a question in resolving the dual luciferase data.
I use pGL3+promoter vector, and I construct my a represstion regulated motif before the SV40 promoter. Then I test the protein repression capacity on this region.
But I found the basic expression level of blank pGL3,pGL3+promoter,pGL3+promoter with egfp co-effected all are very low. But sample which should repress the "represstion regulated motif", their expression is higher than previous three samples I mentioned.
How can I resovle this puzzle?