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pGL3+promoter vector problem

luciferase assays

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#1 lynnmariposa

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Posted 17 March 2018 - 06:17 AM

Hi,everyone.

I have a question in resolving the dual luciferase data.

 

I use pGL3+promoter vector, and I construct my a represstion regulated  motif before the SV40 promoter. Then I test  the protein repression capacity on this region.

 

But I found the basic expression level of blank pGL3,pGL3+promoter,pGL3+promoter with egfp co-effected all are very low. But  sample which should repress the "represstion regulated  motif", their expression is higher than previous three samples I mentioned.

 

How can I resovle this puzzle?

 

 

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#2 bob1

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Posted 23 March 2018 - 08:53 AM

This is a bit late - what exactly are the vectors? I would guess from your post that you are measuring the expression in a vector that is most similar to the pGL3+ promoter construct that you have. Are you normalizing to renilla? if not - you should be.






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