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Visualization of actin using beta actin/phalloidin in neurons

immunohistochemistry actin cytoskeleton neuron fixed tissue

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#1 SJacob



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Posted 15 March 2018 - 09:33 AM

Hi, I was wondering if anyone has labeled actin in neurons with mouse anti-beta actin and/or phalloidin? If you have used both, were there any differences observed, other than beta actin labeling all actin and phalloidin labeling only f-actin?


I have labeled fixed brain tissue from rats with both, and I have noticed phalloidin seems to be labeling the blood vessels while beta actin does not. This suggests that what phalloidin is labeling is not f-actin but was not sure if others have observed anything similar or any additional differences.


Also if you have used either, which would you recommend based on what you observed?


I would appreciate any information!



Also tagged with one or more of these keywords: immunohistochemistry, actin cytoskeleton, neuron, fixed tissue

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