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Virus vs protein purification

virus protein purification

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#1 Thomson



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Posted 12 March 2018 - 09:59 PM

I am looking to purify virus that is incorporated with a protein (a luminescence tag).

So I collected supernatant of infected cells. The virus containing supernatant I then centrifuged at max speed for 90min and discard the supernatant. The pelleted virion I resuspend in PBS and detect the protein using luminescence substrate.


My question is, when I centrifuge as in above condition, can I expect the pelleted is indeed just virion, and any other protein that was expressed by the cells should be in the supernatant after centrifugation. Protein weight much lighter and should not be able to pellet without any precipitation strategy right?

#2 bob1


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Posted 13 March 2018 - 05:29 AM

The pellet will not just be virion - there will be cellular debris in there too. To isolate pure virus you will need to use density gradient isolation.

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