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Measuring concentration of metabolites from MS


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#1 Natasha_



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Posted 28 February 2018 - 07:35 PM

Hi All,

I have observed that the concentration of metabolites present in a living cell is commonly reported in mole/ gram protein.


Could someone explain what gram protein represents?


#2 bob1


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Posted 01 March 2018 - 06:57 AM

gram protein is exactly what it says - a gram of protein. In the example you gave, the mol per gram protein means how many mole of substance X in each gram of protein. 


Note that cells are not entirely made up of protein - there is a lot of water and various lipids in there too, so the experiments determine how much protein there is and use this as a standard for comparison across substances.

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