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How to make DTT?

DTT Western Blot sample buffer

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#1 Mad Researcher

Mad Researcher

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Good

Posted 27 February 2018 - 04:47 PM

I want to make a 2.5 M DTT solution. I have calculated it as follows:

MW of DTT - 154.25 g/mol

The total volume is want is 5 ml.

So, I need to mix 1.925 g in 5 ml of water to make a 2.5 M solution.

 

Is this correct?


Cheers,

Mad Researcher

#2 bob1

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Excellent

Posted 01 March 2018 - 07:03 AM

Short answer: almost. I make it 1.928 g

 

If you show how you calculated and the working - then you can almost always trouble shoot easily.

 

n = v * c

n = 0.005 * 2.5

n = 0.0125

 

m = n * mr

m = 0.0125 * 154.25

m = 1.928







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