my bands spread as a line trough the gel
Posted 29 August 2004 - 09:10 AM
Posted 29 August 2004 - 11:11 PM
Posted 30 August 2004 - 06:54 AM
Posted 30 August 2004 - 08:09 PM
yes, make sure there's no overloading and so leakage from one well into the next. Also make sure that your spacers and combs are the same thickness- if your combs are too thin you will get gel everywhere, and might be loading your samples into the gel, embedding everywhere, rather than neatly into wells.