I heard something interesting about ELISA.
One person was doing ELISA to quantify phosphorylated protein, got abnormally large signal.
Further investigation showed the signal was coming from protease/phosphatase inhibitor not from the sample or matrix effect.
Have you experience the similar issue before?
It is a bit difficult for me to believe and I cannot even find any publication stating that. But I am very curious.
If you happen to know any info, scientific explanation or publication demonstrating that, please let me know!!
Thank you in Advance