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Membrane and whole cell staining

staining immunohistochemistry immunostaining NeuN

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#1 Mad Researcher

Mad Researcher

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Posted 07 February 2018 - 05:24 PM

Hi,

 
I have an image which shows staining for an antibody. It is a membrane bound antibody. 
But I see an empty spot which I assume is the nucleus and the surrounding area where there is staining is the membrane. Am I correct in my assumption? 
 
If it was NeuN antibody, the empty spot/black hole in the image should have been stained and the other region wouldn't have been stained. Please correct me if I am wrong.

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Cheers,

Mad Researcher





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