Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

Membrane and whole cell staining

Started by Mad Researcher, Feb 07 2018 05:24 PM

staining immunohistochemistry immunostaining NeuN

Please log in to reply

No replies to this topic

#1 Mad Researcher

Newbie !!

Active Members
255 posts

15
Good

Posted 07 February 2018 - 05:24 PM

Hi,

I have an image which shows staining for an antibody. It is a membrane bound antibody.

But I see an empty spot which I assume is the nucleus and the surrounding area where there is staining is the membrane. Am I correct in my assumption?

If it was NeuN antibody, the empty spot/black hole in the image should have been stained and the other region wouldn't have been stained. Please correct me if I am wrong.

Attached Thumbnails

Cheers,

Mad Researcher

Back to Protein and Proteomics

Also tagged with one or more of these keywords: staining, immunohistochemistry, immunostaining, NeuN

Protocols and Techniques Forums → Immunology → Visualization of actin using beta actin/phalloidin in neurons Started by SJacob, 15 Mar 2018 immunohistochemistry and 3 more...	0 replies 1,263 views	SJacob 15 Mar 2018
Protocols and Techniques Forums → Histology and Pathology → Intestine tissue falling of slides during H and E staining Started by bryant.keirns, 02 Feb 2018 histology, staining, tissue loss and 1 more...	1 reply 1,604 views	Rnotk 15 Feb 2018
Protocols and Techniques Forums → Tissue and Cell Culture → Neuron dead after immuno Started by Mad Researcher, 22 Jan 2018 immunocytochemistry and 3 more...	8 replies 1,429 views	Mad Researcher 24 Jan 2018
Protocols and Techniques Forums → Immunology → Are HEK293 cells a positive control for wild type p53 expression? Started by sergio_pv, 07 Jul 2016 immunohistochemistry, p53, hek293	1 reply 1,510 views	bob1 07 Jul 2016
Protocols and Techniques Forums → Tissue and Cell Culture → immunofluorescence staining protocol question Started by KhaleesiDany, 02 Oct 2015 immunofluorescence, cell culture and 2 more...	1 reply 1,590 views	bob1 03 Oct 2015