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Whole brain homogenziation for coIP

coIP whole brain homogenization protein

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#1 Mad Researcher

Mad Researcher

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Good

Posted 07 February 2018 - 11:42 AM

Hi,

 

I want to homogenize a whole brain from a P5 or P50 mouse. Currently, I am using a dounce homogenizer to homogenize the brain in Lysis buffer. 

However, I am getting a low yield of proteins from the brain. I sometimes see a large chunk after the lysis. I am unclear if that is causing a low yield of protein and how can I homogenize a whole brain without leaving any large chunk of tissue or membrane.

 

After protein extraction, I follow it up with coIP.


Cheers,

Mad Researcher

#2 bob1

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Excellent

Posted 12 February 2018 - 06:58 AM

Try Potter-Elvehjem  or Tenbroek grinders first for whole tissue. You can follow this with a Dounce if needed.







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