Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Whole brain homogenziation for coIP

coIP whole brain homogenization protein

  • Please log in to reply
1 reply to this topic

#1 Mad Researcher

Mad Researcher

    Newbie !!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 255 posts

Posted 07 February 2018 - 11:42 AM



I want to homogenize a whole brain from a P5 or P50 mouse. Currently, I am using a dounce homogenizer to homogenize the brain in Lysis buffer. 

However, I am getting a low yield of proteins from the brain. I sometimes see a large chunk after the lysis. I am unclear if that is causing a low yield of protein and how can I homogenize a whole brain without leaving any large chunk of tissue or membrane.


After protein extraction, I follow it up with coIP.


Mad Researcher

#2 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,711 posts

Posted 12 February 2018 - 06:58 AM

Try Potter-Elvehjem  or Tenbroek grinders first for whole tissue. You can follow this with a Dounce if needed.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.