There is really strange thing happening in our lab. We are growing S.aureus in minimal media, and when we try to pellet down the cells, we are not able to. Cells sediments in the bottom with spin, however as soon as you pour the supernatant, they get disrupted. We tried taking out sup with pipette also, but there also it gets disrupted. We are using polypropylene bottles. And this is for 1 liter or more than that.
Anybody faced this problem?
Please suggest guys, whats wrong here?