I am new to PCR. Have a problem with specific primer (guinea pig PGP 9.5) in single cell PCR. When I run a gel, I always get smears. But there is no problem with other primers from the same sample. Other colleagues used the primers before me and got good results. I thougt that there was probably contamination of primer mix, or stock primers. So I ordered it again but the problem is still there. Same sample: smears in PGP, normal bands for other primers. Also I got smears in negative water control with PGP, the same water for another primer is clear.
Do someone have any idea what could be wrong?