I want to do a co-IP followed by a Western Blot, however, I am confused.
I use antibody A for doing a coIP. And I want to check the interaction of antibody A with Antibody B.
So, after the coIP steps, I am doing a Western with Antibody B. Is it necessary to do another Western with Antibody A, too?
Or will I see an interaction once I do a Western with Antibody B only? Also, is it necessary to do a loading control antibody too?