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doing a polyA capture twice-does it work?

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#1 molbio1234



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Posted 23 January 2018 - 08:04 PM

Has anyone ever done a poly-A capture twice on mRNA material? Has your recovery been good? I have mRNA enriched material from another source and I need to use it as input into another protocol but the input requires it to be in the protocol specified buffer solution. I would like to recapture the material so that it is eluted in the protocol specified buffer solution (which contains enzymes and some cocktail of reagents, not just a simple buffer). In essence, I will be doing the polyA twice. Has anyone done polyA capture twice and has it worked for you?

#2 bob1


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Posted 24 January 2018 - 01:47 PM

It should work. You will lose some RNA in this process.

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