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SDS precipitation in cold

SDS additive

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#1 Labspace

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Posted 09 January 2018 - 11:07 AM

Hi everyone,

 

Does anyone know how to prevent 2% SDS from precipitating in cold (4*C)? Can I add any additive? Alternatively, is there any SDS alternative that doesn't precipitate at 4*C?

 

Thanks in advance!


Edited by Labspace, 09 January 2018 - 11:08 AM.


#2 Trof

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Posted 10 January 2018 - 07:20 AM

SDS primary function is usualy as a detergent, there may be other alternatives, but depends what you need it for. SDS is an anionic surfactant, while in cold buffers I use, as detergents are used TritonX, Igepal or Tween. But all those are nonionic surfactants.

In things like SDS-PAGE the ionic function is irreplaceable, but for lysis and other uses, it may not be necessary.


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