Hi all,
I've just done normal DNA precipitation in a 50ml falcon (DNA in 10ml H20, 0.1 volume 3M NaAcOH, 2.5 volumes 100% Ethanol) and placed it at -80 for 3h. When I came to take it out it was all super thick. Has anyone seen anything like this before? This never happened to me at -20.
My DNA pellet yield after centrifugation was tiny so I left it at -20 now in hope that whatever did not precipitate at -80 due to the fact that the reaction went weirdly thick, will precipotate at -20 as usual. I'd appreciate any input.