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Amount of protein

protein SDS-PAGE calculation

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#1 Mad Researcher

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Posted 17 December 2017 - 05:09 PM

Hi, 

I have 613 µg/ml of protein. But I want to load 1 mg of protein. Can you please help me with the calculation?

Thanks


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Mad Researcher

#2 bob1

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Excellent

Posted 18 December 2017 - 06:54 AM

v=n/c

 

volume = amount/concentration

 

Keep your units consistent - convert ug to mg (613 ug/ml = 0.613 mg/ml). Make sure you know which volume unit you are using for the final volume too. In this case: ml.

 

v=1/0.613...

 

I'm sure you can get it from there.







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