Hi, I'm planning to observe the protein expressed in living cells using Nano-Glo live cell assay system. It is a bioluminescence substrate.
However, my lab only have 2 fluorescent microscopes, which are:
1) Light Sheet confocal microscope (SP8) Leica Microsystems
2) Confocal laser scanning microscopy (FV1200-IX-KU) Olympus
So my question is: Since luminescent microscope emit light by itself, can we just use fluorescent microscope to view it (without emit any wavelength to excite the reporter)? Or our lab have to buy a bioluminescence microscope?
ps: I've googled online on the above microscopes but they did not state its use regarding bioluminescence imaging.
Edited by Thomson, 13 December 2017 - 05:42 PM.