I am planning to add a tag at the N-terminal of a multipass transmembrane protein.
I wonder if I should add it immediately after the start codon, or after the signal peptide.
From signalP prediction, it seems like it predicted the protein with possible signal peptide btwn amino acid 1-19, but the D value also predicted it as non-signal peptide (results as pasted below).
Does that mean there's likely no signal peptide for the protein sequence?
Anyone used signalP before would really love to hear your input. Thanks
# Measure Position Value Cutoff signal peptide?
max. C 60 0.291
max. Y 20 0.270
max. S 6 0.596
mean S 1-19 0.434
D 1-19 0.336 0.500 NO
Name=Sequence SP='NO' D=0.336 D-cutoff=0.500 Networks=SignalP-TM