I'm working with a kidney disease mouse model, and we want to analyse some urine samples by SDS-PAGE and Coomassie staining. However, I'm concerned that the high salt and urea could interfere with the SDS coating of the proteins and the actual running of the gel.
Is it desirable to centrifuge the samples before ?? 2000g 10 min??
We don't want to interfere to much with the samples (by PEG8000 precipitation a.o.) because we want to be able to use them for other analysis.