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How much protein in S. aureus?

s.aureus protein lysis

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#1 firefly2280

firefly2280

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Posted 22 November 2017 - 06:21 AM

Hi all

 

I want to do a total protein extract from an S. aureus culture but have no idea what density to grow my cells to or how much culture to extract. I need a final conc of 1 mg / ml but want to aviod diluting the extracted protein as much as possible. 

 

Any rough ideas on where to start? 

 

thanks! 







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