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TUNEL help - Immunology - BioForum

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TUNEL help


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2 replies to this topic

#1 flashboy

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Posted 27 August 2004 - 02:55 AM

does anybody know whether or not you need to do a counterstain when you're performing TUNEL????? i seem to remember from years ago that you do but the promega protocol doesn't mention it??

#2 ihcworld

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Posted 29 August 2004 - 07:34 PM

I would suggest a light counterstain using either hematoxylin or methyl green. Please check protocols below:

TUNEL Protocol

Gill's Hematoxylin Solution

Methyl Green Solution

Note LIGHT counterstain. If over do it, positive cells well be covered.

Good luck.

#3 ramakn

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Posted 30 August 2004 - 01:15 PM

I think you need to do a counter stain. You could refer to the manual given by roche-applied for their tunel kits.

All the best




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