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Faint band and primer dimer in PCR

PCR problem

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#1 Bao Duong

Bao Duong


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Posted 16 November 2017 - 07:54 PM

Hi everybody, can you explain my situation ? When i run electrophoresis after pcr, in 3 wells , only 2 of 3 present desired band and all 3 wells have primer-dimer. The primer- dimer is brighter than my desired band. Help me please !!!!!

#2 merlav



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Posted 17 November 2017 - 11:43 AM

trite primer concentration or rise annealing temperature. 

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