Hi everybody, can you explain my situation ? When i run electrophoresis after pcr, in 3 wells , only 2 of 3 present desired band and all 3 wells have primer-dimer. The primer- dimer is brighter than my desired band. Help me please !!!!!
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merlav
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Posted 17 November 2017 - 11:43 AM
trite primer concentration or rise annealing temperature.
Science without religion is lame, religion without science is blind.
Albert Einstein
I am among those who think that science has great beauty. A scientist in his laboratory is not only a technician: he is also a child placed before natural phenomena which impress him like a fairy tale.
Marie Curie
Albert Einstein
I am among those who think that science has great beauty. A scientist in his laboratory is not only a technician: he is also a child placed before natural phenomena which impress him like a fairy tale.
Marie Curie
