We have performed RAPD testing using a published protocol. The assay worked well previously the consistent results. However, the results have become inconsistent recently with different band pattern for the control organisms.
We have checked for the working condition of Taq polymerase, dNTP & MgCl2,
We have use re diluted the primers from the stocks.
Any suggestions are welcome.
Edited by Palitha, 16 November 2017 - 08:09 PM.