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phosphate buffered saline solution for in vivo testing


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#1 labo

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Posted 02 November 2017 - 05:04 PM

what type of phosphate buffered saline should I use for in vivo injections? meaning injecting into humans 


Edited by labo, 02 November 2017 - 05:06 PM.


#2 bob1

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Posted 03 November 2017 - 07:12 AM

Check your ethics approval - there are likely protocols attached. Also check with the clinical side (i.e. Dr's involved in the study)- they should know this or have appropriate diluents available. Having said that PBS is not used clinically as far as I know (not a clinician), much more likely to use Ringers or normal saline. 



#3 labo

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Posted 03 November 2017 - 04:28 PM

bob, thanks for responding. I am injecting a peptide, I don't want to alter the peptide or impede it's effectiveness, what is a good solution to use. 

here is the sequence for the peptide 

YGRKKRRQRRRAQFPGACG



#4 bob1

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Posted 07 November 2017 - 08:48 AM

I would doubt that changing the buffer to a more biologically suitable buffer would alter the peptide effectiveness - again, check with your clinical contacts and read your ethics approvals. Discuss with your PI too, they should have some idea, at least who to ask!



#5 labo

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Posted 07 November 2017 - 03:44 PM

thanks bob






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