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Triton-X-100


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#1 samita

samita

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Posted 26 October 2017 - 01:44 AM

Hi

I have a triton X100 0.5 % in my buffer and its just casuing a smear in my SDS gel and destroy all the bands. I am running the gel at 100 V and 12 %gel. If someone has encounter this problem.

 

best

 

Samita

 



#2 mdfenko

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Posted 27 October 2017 - 04:20 AM

triton displaces sds from the proteins. you will have to remove it if you want to run your protein on sds-page.

 

you can dialyze the sample to remove the triton.


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genius does what it must
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