Im studying gene expression
I want to test a plant extract against bacteria, then studying the gene exprssion changes in bacterial DNA.
Now my question, Im really confused about designing the experiment
If I want to study a gene for the production of lipase that degrade host tissues, someone told me to use a control gene or refrence
how to choose this control gene ?
I thought if I did the cDNA before plant treatment then after treatment and meassuring the quantity or changes of this gene, wouldnt that be enough ? why I should use a control gene ?