Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Long pcr frustration and pron


  • Please log in to reply
No replies to this topic

#1 Angeline

Angeline

    member

  • Active Members
  • Pip
  • 18 posts
0
Neutral

Posted 29 September 2017 - 05:01 AM

I have once managed to amplified a 6.5 kb pcr.
However, the amplification is not reproducible again using the same pcr condition, cDNA samples, primers, high fidelity enzymes. Instead of getting the intended product, a band of less than 2kb base pair is obtained. I?ve tried reextracting the rna, redo reverse transcription, changing dntps, nucleuse free water, new aliquote of primers, new aliquote of enzyme and buffer, but still nothing seem to work. I appreciate All advices to reproduce the 6.5 kb product as it is crucial for my downstream. Thanks a million.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.