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Problem with RNA bands on RNA bleach gel

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#1 MiraZul92

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Posted 26 September 2017 - 02:25 AM

Hi, 


My RNA samples were extracted from human cell lines and stored at -80C.
I used QIAGEN RNeasy mini spin kit for RNA extraction step.
Several days later I ran my RNA samples using RNA Bleach gel and got this result.
The 28S:18S ratio isn't 2:1 
sad.png Does this mean I have to extract new RNA and dump these samples? 

https://imgur.com/a/sp2iD

p.s: I have ran other RNA samples before and got good 28S:18S ratio.

      Just wondering if this batch is good enough for downstream use (qpcr).


-Pupa-


Edited by MiraZul92, 26 September 2017 - 02:26 AM.


#2 ChunlinHuang

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Posted 30 September 2017 - 12:37 AM

RNA Bleach gel is not a particularly rigorous method of detecting RNA quality. If you make RT PCR detect, you can have a try. And you should remember to set negative and positive controls.







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