My RNA samples were extracted from human cell lines and stored at -80C.
I used QIAGEN RNeasy mini spin kit for RNA extraction step.
Several days later I ran my RNA samples using RNA Bleach gel and got this result.
The 28S:18S ratio isn't 2:1
Does this mean I have to extract new RNA and dump these samples?
p.s: I have ran other RNA samples before and got good 28S:18S ratio.
Just wondering if this batch is good enough for downstream use (qpcr).
Edited by MiraZul92, 26 September 2017 - 02:26 AM.