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Mung Bean Nuclease


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#1 bitpas

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Posted 26 August 2004 - 01:39 PM

Hello!

Who from you used Mung-Bean-Nuclease for polishing Restriction-Sites (In my case its a 3'overhang) :blink:

What are/were your experiences? good/bad??

In our institute nobody seems ever to have used it.. :D :)

greeetings!

#2 jadefalcon

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Posted 27 August 2004 - 02:52 AM

yeah, mung bean nuclease works great most of the time, just keep to the manufacturers datasheet.

Only once I had no - read: absolutely no- DNA left after I forgot a MBN digest for over half a day...

mike
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