4 replies to this topic

[spjgjsm]

Hi

About to order a sodium bisulfite kit. I've never used one of these before and was wondering which kit to go for - I have narrowed it down the the MethylEasy kit and the Chemicon CpGENOME kit - which one would you vote for? My considerations are 1) efficiency - i don't want to loose much DNA as I don't have that much to start with, 2) price - as usual my work is on a tightish budget, 3) quality - which kit is actually the best in terms of bisulfite conversion?

Let me know what you all use!

Many thanks

Jon

[pcrman]

I have been using CpGENOME kit (the first of its kind) for 3 years, but never used other kits.
Reliability: good
DNA recovery: good
Price:   $265, for 100 samples

There may be better or cheaper ones, just shop around.

BTW, I am not affiliated with chemicon.

Edited by pcrman, 26 August 2004 - 09:22 AM.

[slab]

Kits may be OK, but I use sodium hydrogen sulfite (SIGMA), HQ, and Wizard DNA Clean-up system (Promega) and they work quite well.

[vkott]

slab, on Sep 15 2004, 11:06 PM, said:

Kits may be OK, but I use sodium hydrogen sulfite (SIGMA), HQ, and Wizard DNA Clean-up system (Promega) and they work quite well.

Can you tell me how to you do it. I can't have a positive result in my PCR and I think the sulphitation is doing bad!!!!
Thanks!!!!

[hslab]

I use EZ DNA methylation kit for a long time, it save time ,only 18 h was needed . and the price is reasonable, 112 dollars for 50 test, It alway give me the beautiful result.