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Is there any significant difference between endogenous/ native promoter and cons


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#1 BIOAWY

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Posted 22 August 2017 - 05:32 PM

Is there any significant difference between endogenous/ native promoter and constitutive promoter (35S) on protein abundance?

 

If a same gene is clone either endogenous promoter or constitutive promoter (35S), Do we get a significantly different protein abundance?



#2 bob1

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Posted 23 August 2017 - 06:05 AM

It depends on the system and the promoter - usually consitituitive promoters will be always on, so they tend to produce a lot of protein, but some of these promoters have low level activity and so don't produce much protein during any given period. Endogenous promoters tend to be more "switchable" so might not always be on, and often have low level expression, but can be high level too.. it all depends on the promoter.

 

While the above is true for mammalian cell based systems, other systems like bacterial and yeast have different results. Bacterial promoters tend to be on all the time, and have relatively high level expression.






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