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Funny shaped PCR curve


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#1 Vyse

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Posted 31 July 2017 - 09:35 AM

Ive been using a custom made mastermix that for some reason results in a qPCR curve that has a baseline lift. I have attached a crude drawing of what the graph looks like. Due to the baselift lift it the CP values are really early, as early as cycle 15.

 

Playing with the initial denaturation temp, denaturation and annealing steps didnt solve the problem.

 

Any help would be greatly appreciated.

 

Thanks

 

anckjnakc.png

 

 



#2 bob1

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Posted 09 August 2017 - 06:07 AM

As you should be reading the values from the exponential part of the curve, baseline lift shouldn't be too much of a problem - it is due to noise when there is little signal usually. 






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