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How to prevent leaking during IP injection in mice?

mouse injection animal

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#1 yl2j

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Posted 25 July 2017 - 01:29 PM

I was doing IP injection in mice. This is how I thought I did the injection: 45 degree insertion at the lower right quadrant of abdomen while mouse is scruff restrained. The plunger was pulled back a little to check that there's no blood before delivery. A couple of the mice I saw that after I remove the needle a drop of liquid starts to form at the site of injection. What am I doing wrong and how can I fix this?

 

Thanks!

 



#2 bob1

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Posted 26 July 2017 - 05:04 AM

Sounds like you are using a needle with a low gauge, try using a smaller diameter needle.







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