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Transfection


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#1 Puppy Singh

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Posted 24 July 2017 - 12:05 PM

Hi guys,

 

Can anyone let me know why transfection decrease after increasing time of incubation with formulation? One reason can be toxicity and is there any other reason too?



#2 bob1

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Posted 25 July 2017 - 05:43 AM

Depends on the method used - For lipofection (e.g. Lipofectamine) the lipospheres are unstable and break down over time. Electroporation reagents tend to be very toxic and the method creates big holes in the membranes. 

 

DNA also gets broken down by external DNases.



#3 Puppy Singh

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Posted 26 July 2017 - 07:01 AM

I'm using liposomes and doing betagal transfection






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