I was planning on running two gels. One with 50ug of protein per well and the other 20ug. I'm looking at a protein where I would need to load decent amount of the protein hence the 50ug.
The loading of the 20ug went smooth, as I was using the 10-well 1.5mm combs from Bio-Rad.
The 50ug not very well. My samples well at 63uL per well and those well take 66uL.
Can I load half of my samples' volume, run the gel until the sample start entering the stacking gel and load the rest?