Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

Help with making a Dimer

Started by kenny17, Jul 09 2017 06:19 AM

cloning ssDNA pGEM3zf+ MSV

Please log in to reply

No replies to this topic

#1 kenny17

member

Members
1 posts

0
Neutral

Posted 09 July 2017 - 06:19 AM

Hi, I need urgent help to make a dimer of Maize Streak Virus (MSV) genome cloned in pGEM3zf+ vector. I am currently having issues with how to digest the plasmid such that it will yield MSV genomes fragments from which I can easily ligate a dimer of the genome into my acceptor plasmid for further processing.

Thanks.

Back to Molecular Cloning

Also tagged with one or more of these keywords: cloning, ssDNA, pGEM3zf+, MSV

Protocols and Techniques Forums → PCR, RT-PCR and Real-Time PCR → problem with PCR amplification of cDNA Started by roshni mohan, 05 Aug 2017 PCR, Cloning, cDNA	4 replies 724 views	roshni mohan 17 Aug 2017
Protocols and Techniques Forums → Molecular Cloning → Molecular cloning into pGhost5 Started by Lana, 07 Apr 2017 cloning	0 replies 986 views	Lana 07 Apr 2017
Protocols and Techniques Forums → Biochemistry → Restriction Digest with pGEMT for TA cloning Started by Mashudu, 27 Mar 2017 cloning, digestion and 3 more...	Hot 14 replies 977 views	Mashudu 28 Apr 2017
Protocols and Techniques Forums → Molecular Biology → Design of <=40bp primers adding restriction sites and also Gibson Assembly o Started by naglemi, 18 Feb 2017 cloning, gibson assembly	1 reply 810 views	bob1 21 Feb 2017
Protocols and Techniques Forums → Molecular Biology → Very low yields for >10kb fragment gel extractions Started by naglemi, 18 Feb 2017 dna, purification, gel extraction and 1 more...	1 reply 763 views	bob1 21 Feb 2017