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Help with making a Dimer

cloning ssDNA pGEM3zf+ MSV

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#1 kenny17

kenny17

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Posted 09 July 2017 - 06:19 AM

Hi, I need urgent help to make a dimer of Maize Streak Virus (MSV) genome cloned in pGEM3zf+ vector. I am currently having issues with how to digest the plasmid such that it will yield MSV genomes fragments from which I can easily ligate a dimer of the genome into my acceptor plasmid for further processing.

 

Thanks.







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