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determination of protein concentation


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#1 samita

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Posted 06 June 2017 - 06:04 AM

I have a protein and its absorbance is 4.8 at 280 nm, and extinction coefficient is 74330. protein is about 100 kda, how do I know what is its protein coencentation??



#2 bob1

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Posted 06 June 2017 - 01:32 PM

Rearrange Beer's law: Abs = extinction * path-length * concentration.






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